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rabbit polyclonal anti pcna antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit polyclonal anti pcna antibody
    CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen <t>(PCNA),</t> a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.
    Rabbit Polyclonal Anti Pcna Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+pcna+antibody/PCNA+Antibody/pmc12685643-86-10-15
    Average 96 stars, based on 6829 article reviews
    rabbit polyclonal anti pcna antibody - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "The CD300c antibody CL7 suppresses tumor growth by regulating the tumor microenvironment in non-small cell lung carcinoma"

    Article Title: The CD300c antibody CL7 suppresses tumor growth by regulating the tumor microenvironment in non-small cell lung carcinoma

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2025.1698857

    CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.
    Figure Legend Snippet: CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.

    Techniques Used: Immunohistochemistry, Marker, Light Microscopy, Software

    Related Articles

    other:

    Article Title: C-kit + cells isolated from developing kidneys are a novel population of stem cells with regenerative potential
    Article Snippet: Scoring for PCNA-positive cells was carried out by counting the number of positive nuclei in four randomly chosen kidney cortex and outer medulla sections using X20 magnification and after applying a rabbit polyclonal PCNA antibody (Santa Cruz).

    Article Title: Antioxidants Block Prostate Cancer in <b> <i>Lady</i> </b> Transgenic Mice
    Article Snippet: The development of chemopreventive agents against prostate cancer would benefit from conclusive evidence of their efficacy in animal models that emulate human disease.. To date there has been little in vivo evidence supporting their preventive capabilities.. The 12T-10 Lady transgenic model spontaneously develops localized prostatic adenocarcinoma and neuroendocrine cancer followed by metastases, recapitulating the natural history of human prostate cancer in many respects.

    Article Title: Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress
    Article Snippet: After electrophoresis, proteins were transferred to nitrocellulose membranes (Protran®, Schleicher &Schuell Bioscience) using blotting apparatus adjusted to 84 mA for 55 min. Membranes were blocked at room temperature with Roti-block solution (Carl Roth GmbH) for 1 hr and then incubated over night at 4°C with primary antibody against each of the candidate cellular proteins (Santa Cruz Biotechnology Inc, Germany: mouse monoclonal β-Actin (1:500), rabbit polyclonal Nrf2 (1:200), Keap1 (1:300), StAR1 (1:350), PCNA (1:350) and 1:300 rabbit polyclonal CAT, Life span Biosciences, Inc. Germany) or exosomal marker proteins (Santa Cruz Biotechnology Inc, Germany: goat polyclonal CYCS (1:350), Alix (1:350) and 1:250 rabbit polyclonal CD63, System BioSciences, USA).

    Incubation:

    Article Title: Inhibitors of NF-κB activity
    Article Snippet: All sections were incubated at 95-100° C. in WCAP reagent (Surgipath) for 30 min, washed and then incubated in 10:1 Methanol:H2O2 for 10 min. .. Sections were then incubated in rabbit polyclonal PCNA (Santa Cruz, Calif.) or antibody detecting NF-κB activation (Chemicon) for 1 h after which immunoreactivity was detected with an Envision assay kit and DAB reagent (DakoCytomation). ..

    Article Title: Heat-induced changes in the expression and localisation of PGC-1α in the mice testis.
    Article Snippet: .. After blocking, slides were incubated with rabbit polyclonal PCNA antibody (1:100, Cat#SC-9707, Santa Cruz), mouse monoclonal GCNA (1:100, Cat#10D9G11, DSHB) and rabbit polyclonal PGC-1α (1:500, kind gift from Daniel P. Kelly, Washington University) overnight at 4°C. .. The slides were washed with PBS and incubated with secondary antibody (for PCNA and PGC-1α, 3 hr at RT with HRP-conjugated goat anti-rabbit IgG, Cat#PI-1000, 1:500, Vector Laboratories) and for GCNA, HRP-conjugated goat anti-mouse IgG (1:500, Cat#SC-2005, Santa Cruz) for 3 hr at RT.

    Article Title: Antiproliferative and antiangiogenic effects of ammonium tetrathiomolybdate in a model of endometriosis.
    Article Snippet: Aims: Copper (Cu) is involved in the endometriosis progression.. Herein, an experimental endometriosis model was used to evaluate whether its chelation with ammonium tetrathiomolybdate (TM) affects the proliferation and angiogenesis in endometriotic-like lesions and the participation of oxidative stress in these processes.. Main methods: Female C57BL/6 mice were divided into three groups: sham-operated mice, endometriosisinduced mice, and TM-treated endometriosis-induced mice.

    Activation Assay:

    Article Title: Inhibitors of NF-κB activity
    Article Snippet: All sections were incubated at 95-100° C. in WCAP reagent (Surgipath) for 30 min, washed and then incubated in 10:1 Methanol:H2O2 for 10 min. .. Sections were then incubated in rabbit polyclonal PCNA (Santa Cruz, Calif.) or antibody detecting NF-κB activation (Chemicon) for 1 h after which immunoreactivity was detected with an Envision assay kit and DAB reagent (DakoCytomation). ..

    Blocking Assay:

    Article Title: Heat-induced changes in the expression and localisation of PGC-1α in the mice testis.
    Article Snippet: .. After blocking, slides were incubated with rabbit polyclonal PCNA antibody (1:100, Cat#SC-9707, Santa Cruz), mouse monoclonal GCNA (1:100, Cat#10D9G11, DSHB) and rabbit polyclonal PGC-1α (1:500, kind gift from Daniel P. Kelly, Washington University) overnight at 4°C. .. The slides were washed with PBS and incubated with secondary antibody (for PCNA and PGC-1α, 3 hr at RT with HRP-conjugated goat anti-rabbit IgG, Cat#PI-1000, 1:500, Vector Laboratories) and for GCNA, HRP-conjugated goat anti-mouse IgG (1:500, Cat#SC-2005, Santa Cruz) for 3 hr at RT.

    Pyrolysis Gas Chromatography:

    Article Title: Heat-induced changes in the expression and localisation of PGC-1α in the mice testis.
    Article Snippet: .. After blocking, slides were incubated with rabbit polyclonal PCNA antibody (1:100, Cat#SC-9707, Santa Cruz), mouse monoclonal GCNA (1:100, Cat#10D9G11, DSHB) and rabbit polyclonal PGC-1α (1:500, kind gift from Daniel P. Kelly, Washington University) overnight at 4°C. .. The slides were washed with PBS and incubated with secondary antibody (for PCNA and PGC-1α, 3 hr at RT with HRP-conjugated goat anti-rabbit IgG, Cat#PI-1000, 1:500, Vector Laboratories) and for GCNA, HRP-conjugated goat anti-mouse IgG (1:500, Cat#SC-2005, Santa Cruz) for 3 hr at RT.

    Saline:

    Article Title: Antiproliferative and antiangiogenic effects of ammonium tetrathiomolybdate in a model of endometriosis.
    Article Snippet: Aims: Copper (Cu) is involved in the endometriosis progression.. Herein, an experimental endometriosis model was used to evaluate whether its chelation with ammonium tetrathiomolybdate (TM) affects the proliferation and angiogenesis in endometriotic-like lesions and the participation of oxidative stress in these processes.. Main methods: Female C57BL/6 mice were divided into three groups: sham-operated mice, endometriosisinduced mice, and TM-treated endometriosis-induced mice.



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    (A) Schematic of a sagittal view of the zebrafish brain highlighting the proximate location of the diencephalic posterior tubercular nucleus (PTN) and innervation of the Mauthner cells (M-cell). (B) Confocal projections of <t>PCNA</t> expression in a representative communal, dominant, and subordinate Tg( dat :egfp) zebrafish. Merged channel also shows DAPI nuclear staining in blue. Arrowheads point to PCNA expressing PTN somata. Scale bar = 20 μm. (C) Summary graph of the fraction of PTN neurons that express PCNA. Symbols represent individual samples; bars represent averages, error bars represent SEM. Dashed lines show pairwise comparison connecting each dominant to its subordinate counterpart. (Com n=6, Dom n= 8, Sub n= 8; One-way ANOVA, P=0.0029 , One-way ANOVA, Tukey’s Multiple Comparison post-test).
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    Image Search Results


    (A) Schematic of a sagittal view of the zebrafish brain highlighting the proximate location of the diencephalic posterior tubercular nucleus (PTN) and innervation of the Mauthner cells (M-cell). (B) Confocal projections of PCNA expression in a representative communal, dominant, and subordinate Tg( dat :egfp) zebrafish. Merged channel also shows DAPI nuclear staining in blue. Arrowheads point to PCNA expressing PTN somata. Scale bar = 20 μm. (C) Summary graph of the fraction of PTN neurons that express PCNA. Symbols represent individual samples; bars represent averages, error bars represent SEM. Dashed lines show pairwise comparison connecting each dominant to its subordinate counterpart. (Com n=6, Dom n= 8, Sub n= 8; One-way ANOVA, P=0.0029 , One-way ANOVA, Tukey’s Multiple Comparison post-test).

    Journal: bioRxiv

    Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

    doi: 10.64898/2026.01.27.701991

    Figure Lengend Snippet: (A) Schematic of a sagittal view of the zebrafish brain highlighting the proximate location of the diencephalic posterior tubercular nucleus (PTN) and innervation of the Mauthner cells (M-cell). (B) Confocal projections of PCNA expression in a representative communal, dominant, and subordinate Tg( dat :egfp) zebrafish. Merged channel also shows DAPI nuclear staining in blue. Arrowheads point to PCNA expressing PTN somata. Scale bar = 20 μm. (C) Summary graph of the fraction of PTN neurons that express PCNA. Symbols represent individual samples; bars represent averages, error bars represent SEM. Dashed lines show pairwise comparison connecting each dominant to its subordinate counterpart. (Com n=6, Dom n= 8, Sub n= 8; One-way ANOVA, P=0.0029 , One-way ANOVA, Tukey’s Multiple Comparison post-test).

    Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

    Techniques: Expressing, Staining, Comparison

    (A) PC1 and PC2 are the first and the second principal components excluding social isolates. (B) Eigenvalues of the principal components excluding social isolates showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. (C) PC1 and PC2 are the first and the second Principal components excluding PCNA as a component. (D) Eigenvalues of the principal components excluding PNCA as a component showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. In A and C, points represent individual samples (n= 6 per social condition), different colors and symbols represent different groups. Ellipses represent 68% confidence intervals of core regions. Loading vectors represent original variables, the directions of arrows represent correlation between original variable and principal components, lengths represent association of original data to principal components. In B and D components are listed in descending order (highest to the lowest).

    Journal: bioRxiv

    Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

    doi: 10.64898/2026.01.27.701991

    Figure Lengend Snippet: (A) PC1 and PC2 are the first and the second principal components excluding social isolates. (B) Eigenvalues of the principal components excluding social isolates showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. (C) PC1 and PC2 are the first and the second Principal components excluding PCNA as a component. (D) Eigenvalues of the principal components excluding PNCA as a component showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. In A and C, points represent individual samples (n= 6 per social condition), different colors and symbols represent different groups. Ellipses represent 68% confidence intervals of core regions. Loading vectors represent original variables, the directions of arrows represent correlation between original variable and principal components, lengths represent association of original data to principal components. In B and D components are listed in descending order (highest to the lowest).

    Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

    Techniques:

    Representative of H&E and PCNA IHC with TM concentrations in tumor and different organs (A) H&E staining of untreated and NP TM-treated KPC-cell-derived xenograft tumor (KPC-CDX) sections. Scale bars, 50 μm. (B) PCNA immunohistochemistry in untreated and NP TM-treated KPC-CDX sections. Bar graph showing the statistical difference of PCNA in untreated and untreated samples. Data represent mean ± SD. n = 5. Scale bars, 50 μm. (C) H&E staining in different organs of KPC-CDX mice upon NP TM treatment. 40×. Scale bars, 50 μm.

    Journal: Molecular Therapy Oncology

    Article Title: Targeted nanoencapsulation of tunicamycin reduces toxicity while improving its therapeutic effectiveness in pancreatic cancer cells

    doi: 10.1016/j.omton.2025.201047

    Figure Lengend Snippet: Representative of H&E and PCNA IHC with TM concentrations in tumor and different organs (A) H&E staining of untreated and NP TM-treated KPC-cell-derived xenograft tumor (KPC-CDX) sections. Scale bars, 50 μm. (B) PCNA immunohistochemistry in untreated and NP TM-treated KPC-CDX sections. Bar graph showing the statistical difference of PCNA in untreated and untreated samples. Data represent mean ± SD. n = 5. Scale bars, 50 μm. (C) H&E staining in different organs of KPC-CDX mice upon NP TM treatment. 40×. Scale bars, 50 μm.

    Article Snippet: Anti-rabbit IgG (# 70745) was purchased from Cell Signaling, USA; anti-mouse IgG (# sc-516102) from Santa Cruz, Inc, USA; CCN1 polyclonal antibody (#26689-1-AP) and Neuropilin-1 (NRP-1) from ProteinTech, USA; and pSTAT3 polyclonal antibody (#9145) and STAT3 polyclonal antibody, peIF4E and EIF4E antibodies, and PCNA rabbit polyclonal antibody (#13110T) from Cell Signaling, Inc., USA.

    Techniques: Staining, Derivative Assay, Immunohistochemistry

    CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.

    Journal: Frontiers in Oncology

    Article Title: The CD300c antibody CL7 suppresses tumor growth by regulating the tumor microenvironment in non-small cell lung carcinoma

    doi: 10.3389/fonc.2025.1698857

    Figure Lengend Snippet: CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.

    Article Snippet: The sections were then incubated overnight at 4°C with a rabbit polyclonal anti-PCNA antibody (1:200, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Immunohistochemistry, Marker, Light Microscopy, Software